Fibronectin Kit ELISA
Aperçu rapide pour Fibronectin Kit ELISA (ABIN2042468)
Antigène
Voir toutes Fibronectin Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 6 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 15 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-Fibronectin, Rat
Lyophilized recombinant Fibronectin, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Fibronectin ELISA Kit
High Sensitivity Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivité: Boeuf (Vache) Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivité: Humain Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivité: Souris Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivité: Rat Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Fibronectin ELISA KitReactivité: Rat Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivité: Porc Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin ELISA KitReactivité: Humain Colorimetric Competition ELISA 0.781-50 μg/mL Plasma, Serum
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Rat Fibronectin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides.1 These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris.2 Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.3 The standard used in this kit is isolated from rat plasma with the molecular mass of 200-250kD.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- Fibronectin
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ID gène
- 25661
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UniProt
- P04937
Antigène Voir toutes Fibronectin Kits ELISA
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