Lipocalin 2 Kit ELISA

N° du produit ABIN2042580

Ce kit ELISA Rat Lipocalin 2 est un kit ELISA Colorimetric conçu pour quantifier Rat Lipocalin 2.

Aperçu rapide pour Lipocalin 2 Kit ELISA (ABIN2042580)

Antigène: Lipocalin 2 (LCN2)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6-10000 pg/mL

Application: ELISA

Détail du produit Lipocalin 2 Kit ELISA

Seuil minimal de détection: 6 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients: 96-well plate pre-coated with anti-Lipocalin-2/NGAL, Rat
Lyophilized recombinant Lipocalin-2/NGAL, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Rat Lipocalin-2/NGAL in cell culture supernates, serum, plasma (heparin) and urine.Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kD.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Lipocalin 2 (LCN2)

Autre désignation: Lipocalin-2/NGAL

ID gène: 170496

UniProt: P30152

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis