Osteopontin Kit ELISA

N° du produit ABIN2042624

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Osteopontin.

Aperçu rapide pour Osteopontin Kit ELISA (ABIN2042624)

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6-10000 pg/mL

Application: ELISA

Détail du produit Osteopontin Kit ELISA

Seuil minimal de détection: 6 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients: 96-well plate pre-coated with anti-OPN, Human
Lyophilized recombinant OPN, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Human OPN in cell culture supernates, serum, plasma (heparin, EDTA) and urine and human milk.Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis.1 Osteopontin is presumably involved in stone formation as stone matrix.2 The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4.3 The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9kD. As a result of glycosylation, the molecular mass is 60-65kD.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Autre désignation: OPN

ID gène: 6696

UniProt: P10451

Pathways: Regulation of Cell Size