Soluble E-Selectin Kit ELISA

N° du produit ABIN2042658

Le kit ELISA Soluble E-Selectin (ABIN2042658) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Soluble E-Selectin.

Aperçu rapide pour Soluble E-Selectin Kit ELISA (ABIN2042658)

Antigène: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 5-8000 pg/mL

Application: ELISA

Détail du produit Soluble E-Selectin Kit ELISA

Seuil minimal de détection: 5 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 4 pg/mL

Ingrédients: 96-well plate pre-coated with anti-sE-Selectin, Human
Lyophilized recombinant sE-Selectin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Human E-Selectin in cell culture supernates, serum and plasma (heparin, citrate).E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM).3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4 The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6kD.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Autre désignation: sE-Selectin

ID gène: 6401

UniProt: P16581