FAP Kit ELISA

N° du produit ABIN2042661

Ce kit ELISA Humain FAP est un kit ELISA Colorimetric conçu pour quantifier Humain FAP.

Aperçu rapide pour FAP Kit ELISA (ABIN2042661)

Antigène: FAP (Fibroblast Activation Protein, alpha (FAP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Détail du produit FAP Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients: 96-well plate pre-coated with anti-Seprase/FAP, Human
Lyophilized recombinant Seprase/FAP, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Human Seprase/FAP in cell culture supernates, serum and plasma (heparin, EDTA).FAP (Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: FAP (Fibroblast Activation Protein, alpha (FAP))

Autre désignation: Seprase/FAP

ID gène: 2191

UniProt: Q12884

Pathways: Tube Formation