TIMP1 Kit ELISA
Aperçu rapide pour TIMP1 Kit ELISA (ABIN2042680)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 6 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 5 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-TIMP-1, Human
Lyophilized recombinant TIMP-1, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Human TIMP-1 in cell culture supernates, serum and plasma (heparin, EDTA) and saliva.The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is natural TIMP-1 with the molecular mass of 22kD.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
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Autre désignation
- TIMP-1
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ID gène
- 7076
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UniProt
- P01033
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