TIMP1 Kit ELISA

N° du produit ABIN2042681

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat TIMP1.

Aperçu rapide pour TIMP1 Kit ELISA (ABIN2042681)

Antigène: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Détail du produit TIMP1 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 3 pg/mL

Ingrédients: 96-well plate pre-coated with anti-TIMP-1, Rat
Lyophilized recombinant TIMP-1, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Rat TIMP-1 in cell culture supernates, serum and plasma (heparin, EDTA).The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5kD. As a result of glycosylation, the molecular mass is 32-34kD.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Autre désignation: TIMP-1

ID gène: 116510

UniProt: P30120