PLAU Kit ELISA
Aperçu rapide pour PLAU Kit ELISA (ABIN2042715)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 62.5 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 5 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-uPA/PLAU, Human
Lyophilized recombinant uPA/PLAU, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Human uPA/PLAU in cell culture supernates, serum and plasma (heparin, EDTA).Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18kD and 33kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- PLAU (Plasminogen Activator, Urokinase (PLAU))
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Autre désignation
- uPA/PLAU
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ID gène
- 5328
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UniProt
- P00749
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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