VCAM1 Kit ELISA

N° du produit ABIN2042717

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris VCAM1.

Aperçu rapide pour VCAM1 Kit ELISA (ABIN2042717)

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6-10000 pg/mL

Application: ELISA

Détail du produit VCAM1 Kit ELISA

Seuil minimal de détection: 6 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients: 96-well plate pre-coated with anti-VCAM-1, Mouse
Lyophilized recombinant VCAM-1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Mouse VCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9kD. As a result of glycosylation, the molecular mass of 120kD is revealed by SDS-PAGE.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: VCAM-1

ID gène: 22329

UniProt: P29533

Pathways: Carbohydrate Homeostasis