HSD11B1L Kit ELISA

N° du produit ABIN2042731

Kit ELISA HSD11B1L Humain, Colorimetric test pour la quantification de Humain HSD11B1L.

Aperçu rapide pour HSD11B1L Kit ELISA (ABIN2042731)

Antigène: HSD11B1L (Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit HSD11B1L Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.065 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HSD11B1L (Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))

Autre désignation: 11-beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3)