Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris .
N° du produit ABIN2042735
Aperçu rapide pour Proteasome 20S Kit ELISA (ABIN2042735)
Antigène
Proteasome 20S
Reactivité
Souris
Méthode de détection
Colorimetric
Type de méthode
Competition ELISA
Gamme de detection
370.37-30000 ng/mL
Application
ELISA
Seuil minimal de détection
370.37 ng/mL
Marque
BioAssay™
Sensibilité
< 135.6 ng/mL
Plaque
Pre-coated
Protocole
20S-Proteasome (20S-PSM) BioAssay-ELISA Kit (Mouse) employs the competitive inhibition enzyme immunoassay technique. An antibody specific for 20S-Proteasome (20S-PSM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled 20S-Proteasome (20S-PSM) and unlabeled 20S-Proteasome (20S-PSM) (Standards or samples) with the pre-coated antibody specific for 20S-Proteasome (20S-PSM). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of 20S-Proteasome (20S-PSM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 20S-Proteasome (20S-PSM) in the sample.