ADCY10 Kit ELISA

N° du produit ABIN2042777

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Rat ADCY10.

Aperçu rapide pour ADCY10 Kit ELISA (ABIN2042777)

Antigène: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit ADCY10 Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.125 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Adenylate Cyclase 10, Soluble (ADCY10) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 10, Soluble (ADCY10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 10, Soluble (ADCY10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 10, Soluble (ADCY10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Autre désignation: Adenylate Cyclase 10, Soluble (ADCY10)

Pathways: cAMP Metabolic Process