ADH1 Kit ELISA

N° du produit ABIN2042801

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris ADH1.

Aperçu rapide pour ADH1 Kit ELISA (ABIN2042801)

Antigène: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12-200 ng/mL

Application: ELISA

Détail du produit ADH1 Kit ELISA

Seuil minimal de détection: 3.12 ng/mL

Sensibilité: < 1.32 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Alcohol Dehydrogenase 1 (ADH1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 1 (ADH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 1 (ADH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 1 (ADH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alcohol Dehydrogenase 1 (ADH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Autre désignation: Alcohol Dehydrogenase 1 (ADH1)