alpha Fetoprotein Kit ELISA

N° du produit ABIN2042821

Kit ELISA Singe alpha Fetoprotein, test Colorimetric pour la quantification de Singe alpha Fetoprotein.

Aperçu rapide pour alpha Fetoprotein Kit ELISA (ABIN2042821)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Singe

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit alpha Fetoprotein Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.118 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Alpha-Fetoprotein (aFP) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Alpha-Fetoprotein (aFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Pathways: C21-Steroid Hormone Metabolic Process