Alpha-amylase 1 Kit ELISA

N° du produit ABIN2042833

Kit ELISA Rat Alpha-amylase 1, test Colorimetric pour la quantification de Rat Alpha-amylase 1.

Aperçu rapide pour Alpha-amylase 1 Kit ELISA (ABIN2042833)

Antigène: Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Détail du produit Alpha-amylase 1 Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Sensibilité: < 0.28 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Amylase Alpha 1, Salivary (AMY1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amylase Alpha 1, Salivary (AMY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amylase Alpha 1, Salivary (AMY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amylase Alpha 1, Salivary (AMY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Amylase Alpha 1, Salivary (AMY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Alpha-amylase 1 (AMY1) (Amylase 1, Salivary (AMY1))

Autre désignation: Amylase alpha 1, Salivary (AMY1)