Anti-Myeloperoxidase Antibody (MPO pANCA) Kit ELISA

N° du produit ABIN2042907

Le kit ELISA Anti-Myeloperoxidase Antibody (ABIN2042907) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Anti-Myeloperoxidase Antibody.

Aperçu rapide pour Anti-Myeloperoxidase Antibody (MPO pANCA) Kit ELISA (ABIN2042907)

Antigène: Anti-Myeloperoxidase Antibody (MPO pANCA)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12-200 ng/mL

Application: ELISA

Détail du produit

Seuil minimal de détection: 3.12 ng/mL

Sensibilité: < 1.12 ng/mL

Ingrédients: A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Assay Diluent A, 1x10 mL
F: TMB Substrate, 1x0 mL
G: Stop Solution, 1x0 mL
H: Wash Buffer, 30X, 1x20 mL

Information d'application

Plaque: Pre-coated

Protocole: The Human Anti-Myeloperoxidase Antibody (Anti-MPO) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-MPO in human serum, plasma and other biological fluids.The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-MPO will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-MPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Anti-Myeloperoxidase Antibody (MPO pANCA)

Classe de substances: Antibody