BHMT Kit ELISA

N° du produit ABIN2043009

Ce kit ELISA Humain BHMT est un kit ELISA Colorimetric conçu pour quantifier Humain BHMT.

Aperçu rapide pour BHMT Kit ELISA (ABIN2043009)

Antigène: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit BHMT Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.124 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Betaine Homocysteine Methyltransferase (BHMT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Autre désignation: Betaine Homocysteine Methyltransferase (BHMT)

Pathways: Methionine Biosynthetic Process