CASR Kit ELISA

N° du produit ABIN2043047

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris CASR.

Aperçu rapide pour CASR Kit ELISA (ABIN2043047)

Antigène: CASR (Calcium-Sensing Receptor (CASR))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit CASR Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.057 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Calcium Sensing Receptor (CASR) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Sensing Receptor (CASR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Sensing Receptor (CASR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Sensing Receptor (CASR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Calcium Sensing Receptor (CASR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CASR (Calcium-Sensing Receptor (CASR))

Autre désignation: Calcium Sensing Receptor (CASR)

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis