Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain CA 19-5.
N° du produit ABIN2043065
Aperçu rapide pour CA 19-5 Kit ELISA (ABIN2043065)
Antigène
CA 19-5
(Carbohydrate Antigen 19-5 (CA 19-5))
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Competition ELISA
Gamme de detection
2.47-200 ng/mL
Application
ELISA
Seuil minimal de détection
2.47 ng/mL
Marque
BioAssay™
Sensibilité
< 0.86 ng/mL
Plaque
Pre-coated
Protocole
Carbohydrate Antigen 19-5 (CA19-5) BioAssay-ELISA Kit (Human) employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Carbohydrate Antigen 19-5 (CA19-5) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Carbohydrate Antigen 19-5 (CA19-5) and unlabeled Carbohydrate Antigen 19-5 (CA19-5) (Standards or samples) with the pre-coated antibody specific for Carbohydrate Antigen 19-5 (CA19-5). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample.