FLASH Kit ELISA

N° du produit ABIN2043079

Kit ELISA Rat FLASH, test Colorimetric pour la quantification de Rat FLASH.

Aperçu rapide pour FLASH Kit ELISA (ABIN2043079)

Antigène: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit FLASH Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Marque: BioAssay™

Sensibilité: < 0.136 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Caspase 8 Associated Protein 2 (CASP8AP2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 Associated Protein 2 (CASP8AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 Associated Protein 2 (CASP8AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8 Associated Protein 2 (CASP8AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Caspase 8 Associated Protein 2 (CASP8AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Autre désignation: Caspase 8 Associated Protein 2 (CASP8AP2)

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity