CERK Kit ELISA

N° du produit ABIN2043115

Kit ELISA Humain CERK, test Colorimetric pour la quantification de Humain CERK.

Aperçu rapide pour CERK Kit ELISA (ABIN2043115)

Antigène: CERK (Ceramide Kinase (CERK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12-200 ng/mL

Application: ELISA

Détail du produit CERK Kit ELISA

Seuil minimal de détection: 3.12 ng/mL

Sensibilité: < 1.44 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Ceramide Kinase (CERK) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceramide Kinase (CERK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CERK (Ceramide Kinase (CERK))