CHRNB1 Kit ELISA
Aperçu rapide pour CHRNB1 Kit ELISA (ABIN2043147)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 78.12 pg/mL
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Sensibilité
- < 31 pg/mL
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Plaque
- Pre-coated
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Protocole
- Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))
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Autre désignation
- Cholinergic Receptor, Nicotinic, beta 1 (CHRNb1)
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Pathways
- Synaptic Membrane
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