Complement C4d Kit ELISA
Aperçu rapide pour Complement C4d Kit ELISA (ABIN2043203)
Antigène
Tous les produits Complement C4d (C4d)Reactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 9.37 pg/mL
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Sensibilité
- < 4.2 pg/mL
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Plaque
- Pre-coated
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Protocole
- Complement Component 4d (C4d) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4d (C4d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 4d (C4d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 4d (C4d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 4d (C4d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- Complement C4d (C4d) (Complement Component C4d (C4d))
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Autre désignation
- Complement Component 4d (C4d)
Antigène Tous les produits Complement C4d (C4d)
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