CDK16 Kit ELISA
Aperçu rapide pour CDK16 Kit ELISA (ABIN2043251)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 31.25 pg/mL
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Sensibilité
- < 12.6 pg/mL
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Plaque
- Pre-coated
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Protocole
- Cyclin Dependent Kinase 16 (CDK16) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 16 (CDK16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 16 (CDK16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 16 (CDK16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cyclin Dependent Kinase 16 (CDK16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- CDK16 (Cyclin-Dependent Kinase 16 (CDK16))
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Autre désignation
- Cyclin Dependent Kinase 16 (CDK16)
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Pathways
- Hormone Transport
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