DDX5 Kit ELISA

N° du produit ABIN2043291

Le kit ELISA DDX5 (ABIN2043291) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain DDX5.

Aperçu rapide pour DDX5 Kit ELISA (ABIN2043291)

Antigène: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit DDX5 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.062 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: DEAD Box Polypeptide 5 (DDX5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEAD Box Polypeptide 5 (DDX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DEAD Box Polypeptide 5 (DDX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEAD Box Polypeptide 5 (DDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of DEAD Box Polypeptide 5 (DDX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Autre désignation: DEAD Box Polypeptide 5 (DDX5)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus