DEFB112 Kit ELISA

N° du produit ABIN2043303

Ce kit ELISA Humain DEFB112 est un kit ELISA Colorimetric conçu pour quantifier Humain DEFB112.

Aperçu rapide pour DEFB112 Kit ELISA (ABIN2043303)

Antigène: DEFB112 (Defensin, beta 112 (DEFB112))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12-5000 pg/mL

Application: ELISA

Détail du produit DEFB112 Kit ELISA

Seuil minimal de détection: 78.12 pg/mL

Sensibilité: < 29.4 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Defensin Beta 112 (DEFb112) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 112 (DEFb112). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 112 (DEFb112). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 112 (DEFb112), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 112 (DEFb112) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: DEFB112 (Defensin, beta 112 (DEFB112))

Autre désignation: Defensin beta 112 (DEFb112)