DEFB124 Kit ELISA

N° du produit ABIN2043305

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain DEFB124.

Aperçu rapide pour DEFB124 Kit ELISA (ABIN2043305)

Antigène: DEFB124 (Defensin, beta 124 (DEFB124))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62-1000 pg/mL

Application: ELISA

Détail du produit DEFB124 Kit ELISA

Seuil minimal de détection: 15.62 pg/mL

Sensibilité: < 6.4 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Defensin Beta 124 (DEFb124) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 124 (DEFb124). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 124 (DEFb124). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 124 (DEFb124), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 124 (DEFb124) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: DEFB124 (Defensin, beta 124 (DEFB124))

Autre désignation: Defensin beta 124 (DEFb124)