beta 2 Defensin Kit ELISA

N° du produit ABIN2043311

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Boeuf (Vache) beta 2 Defensin.

Aperçu rapide pour beta 2 Defensin Kit ELISA (ABIN2043311)

Antigène: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Détail du produit beta 2 Defensin Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Sensibilité: < 22.6 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Defensin Beta 2 (DEFb2) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 2 (DEFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 2 (DEFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 2 (DEFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Defensin Beta 2 (DEFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Autre désignation: Defensin beta 2 (DEFb2)