DSE Kit ELISA

N° du produit ABIN2043329

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain DSE.

Aperçu rapide pour DSE Kit ELISA (ABIN2043329)

Antigène: DSE (Dermatan Sulfate Epimerase (DSE))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit DSE Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.114 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Dermatan Sulfate Epimerase (DSE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatan Sulfate Epimerase (DSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatan Sulfate Epimerase (DSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatan Sulfate Epimerase (DSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dermatan Sulfate Epimerase (DSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: DSE (Dermatan Sulfate Epimerase (DSE))

Pathways: Glycosaminoglycan Metabolic Process