AKR1C2 Kit ELISA

N° du produit ABIN2043343

Kit ELISA AKR1C2 Humain, Colorimetric test pour la quantification de Humain AKR1C2.

Aperçu rapide pour AKR1C2 Kit ELISA (ABIN2043343)

Antigène: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit AKR1C2 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.061 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Dihydrodiol Dehydrogenase 2 (DDH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrodiol Dehydrogenase 2 (DDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dihydrodiol Dehydrogenase 2 (DDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Autre désignation: Dihydrodiol Dehydrogenase 2 (DDH2)

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process