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DPYS Kit ELISA

Ce kit ELISA Humain DPYS est un kit ELISA Colorimetric conçu pour quantifier Humain DPYS.
N° du produit ABIN2043345

Aperçu rapide pour DPYS Kit ELISA (ABIN2043345)

Antigène

Voir toutes DPYS Kits ELISA
DPYS (Dihydropyrimidinase (DPYS))

Reactivité

  • 1
  • 1
Humain

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

0.78-50 ng/mL

Application

ELISA
  • Seuil minimal de détection

    0.78 ng/mL

    Sensibilité

    < 0.31 ng/mL
  • Plaque

    Pre-coated

    Protocole

    Dihydropyrimidinase (DPYS) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase (DPYS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidinase (DPYS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase (DPYS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dihydropyrimidinase (DPYS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Stock

    4 °C/-20 °C
  • Antigène Voir toutes DPYS Kits ELISA

    DPYS (Dihydropyrimidinase (DPYS))
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