CELA2A Kit ELISA

N° du produit ABIN2043389

Détail du produit CELA2A Kit ELISA

Antigène: CELA2A (Chymotrypsin-Like Elastase Family, Member 2A (CELA2A))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 12.8 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Elastase 2A (ELA2A) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 2A (ELA2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 2A (ELA2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 2A (ELA2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Elastase 2A (ELA2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CELA2A (Chymotrypsin-Like Elastase Family, Member 2A (CELA2A))

Autre désignation: Elastase 2A (ELA2A) (CELA2A Produits)

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