ETFB Kit ELISA

N° du produit ABIN2043393

Le kit ELISA ETFB (ABIN2043393) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain ETFB.

Aperçu rapide pour ETFB Kit ELISA (ABIN2043393)

Antigène: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit ETFB Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.112 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Electron Transfer Flavoprotein Beta Polypeptide (ETFb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Beta Polypeptide (ETFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Beta Polypeptide (ETFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Electron Transfer Flavoprotein Beta Polypeptide (ETFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: ETFB (Electron-Transfer-Flavoprotein, beta Polypeptide (ETFB))

Autre désignation: Electron Transfer Flavoprotein beta Polypeptide (ETFb)