EDC1
(Enhancer Of mRNA Decapping Protein 1 (EDC1))
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.15-10 ng/mL
Application
ELISA
Seuil minimal de détection
0.15 ng/mL
Sensibilité
< 0.045 ng/mL
Plaque
Pre-coated
Protocole
Enhancer Of mRNA Decapping Protein 1 (EDC1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enhancer Of mRNA Decapping Protein 1 (EDC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enhancer Of mRNA Decapping Protein 1 (EDC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enhancer Of mRNA Decapping Protein 1 (EDC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Enhancer Of mRNA Decapping Protein 1 (EDC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.