EPHX4 Kit ELISA

N° du produit ABIN2043409

Kit ELISA EPHX4 Souris, Colorimetric test pour la quantification de Souris EPHX4.

Aperçu rapide pour EPHX4 Kit ELISA (ABIN2043409)

Antigène: EPHX4 (Epoxide Hydrolase 4 (EPHX4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Détail du produit EPHX4 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Sensibilité: < 27.2 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Epoxide Hydrolase 4 (EPHX4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epoxide Hydrolase 4 (EPHX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epoxide Hydrolase 4 (EPHX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epoxide Hydrolase 4 (EPHX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Epoxide Hydrolase 4 (EPHX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: EPHX4 (Epoxide Hydrolase 4 (EPHX4))