FAM135B Kit ELISA

N° du produit ABIN2043433

Kit ELISA FAM135B Souris, Colorimetric test pour la quantification de Souris FAM135B.

Aperçu rapide pour FAM135B Kit ELISA (ABIN2043433)

Antigène: FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit FAM135B Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.057 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Family With Sequence Similarity 135, Member B (FAM135B) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 135, Member B (FAM135B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Family With Sequence Similarity 135, Member B (FAM135B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))