Frataxin Kit ELISA

N° du produit ABIN2043505

Kit ELISA Souris Frataxin, test Colorimetric pour la quantification de Souris Frataxin.

Aperçu rapide pour Frataxin Kit ELISA (ABIN2043505)

Antigène: Frataxin (FXN)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit Frataxin Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.055 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Frataxin (FXN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frataxin (FXN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frataxin (FXN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frataxin (FXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Frataxin (FXN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Frataxin (FXN)

Pathways: Transition Metal Ion Homeostasis, Regulation of Cell Size