GPR109B Kit ELISA

N° du produit ABIN2043521

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain GPR109B.

Aperçu rapide pour GPR109B Kit ELISA (ABIN2043521)

Antigène: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit GPR109B Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.061 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: G Protein Coupled Receptor 109B (GPR109B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 109B (GPR109B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 109B (GPR109B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 109B (GPR109B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of G Protein Coupled Receptor 109B (GPR109B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Autre désignation: G-Protein Coupled Receptor 109B (GPR109B)