GSAP Kit ELISA

N° du produit ABIN2043535

Kit ELISA GSAP Rat, Colorimetric test pour la quantification de Rat GSAP.

Aperçu rapide pour GSAP Kit ELISA (ABIN2043535)

Antigène: GSAP (PION) (Pigeon Homolog (PION))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit GSAP Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.113 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Gamma-Secretase Activating Protein (gSAP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Secretase Activating Protein (gSAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Secretase Activating Protein (gSAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Secretase Activating Protein (gSAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Gamma-Secretase Activating Protein (gSAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: GSAP (PION) (Pigeon Homolog (PION))

Autre désignation: gamma-Secretase Activating Protein (gSAP)