MBOAT4 Kit ELISA

N° du produit ABIN2043541

Kit ELISA MBOAT4 Humain, Colorimetric test pour la quantification de Humain MBOAT4.

Aperçu rapide pour MBOAT4 Kit ELISA (ABIN2043541)

Antigène: MBOAT4 (Membrane Bound O-Acyltransferase Domain Containing 4 (MBOAT4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Détail du produit MBOAT4 Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Sensibilité: < 0.26 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Ghrelin-O-Acyltransferase (GOAT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ghrelin-O-Acyltransferase (GOAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ghrelin-O-Acyltransferase (GOAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ghrelin-O-Acyltransferase (GOAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ghrelin-O-Acyltransferase (GOAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: MBOAT4 (Membrane Bound O-Acyltransferase Domain Containing 4 (MBOAT4))

Autre désignation: Ghrelin-O-Acyltransferase (GOAT)

Pathways: Peptide Hormone Metabolism