GAA Kit ELISA

N° du produit ABIN2043555

Ce kit ELISA Souris GAA est un kit ELISA Colorimetric conçu pour quantifier Souris GAA.

Aperçu rapide pour GAA Kit ELISA (ABIN2043555)

Antigène: GAA (Glucosidase, Alpha, Acid (GAA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Application: ELISA

Détail du produit GAA Kit ELISA

Seuil minimal de détection: 31.25 pg/mL

Sensibilité: < 14.3 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Glucosidase Alpha, Acid (GaA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Alpha, Acid (GaA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucosidase Alpha, Acid (GaA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucosidase Alpha, Acid (GaA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucosidase Alpha, Acid (GaA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: GAA (Glucosidase, Alpha, Acid (GAA))

Autre désignation: Glucosidase Alpha, Acid (GaA)

Pathways: Cellular Glucan Metabolic Process