HSPA1B Kit ELISA

N° du produit ABIN2043613

Kit ELISA Boeuf (Vache) HSPA1B, test Colorimetric pour la quantification de Boeuf (Vache) HSPA1B.

Aperçu rapide pour HSPA1B Kit ELISA (ABIN2043613)

Antigène: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Détail du produit HSPA1B Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Sensibilité: < 0.53 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Heat Shock 70kDa Protein 1B (HSPA1B) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock 70kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))