ChT Kit ELISA

N° du produit ABIN2043643

Kit ELISA Rat ChT, test Colorimetric pour la quantification de Rat ChT.

Aperçu rapide pour ChT Kit ELISA (ABIN2043643)

Antigène: ChT (High Affinity Choline Transporter (ChT))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12-5000 pg/mL

Application: ELISA

Détail du produit ChT Kit ELISA

Seuil minimal de détection: 78.12 pg/mL

Sensibilité: < 28 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: High Affinity Choline Transporter (ChT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Affinity Choline Transporter (ChT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Affinity Choline Transporter (ChT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Affinity Choline Transporter (ChT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of High Affinity Choline Transporter (ChT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: ChT (High Affinity Choline Transporter (ChT))