IgG Kit ELISA

N° du produit ABIN2043661

Kit ELISA IgG Boeuf (Vache), Colorimetric test pour la quantification de Boeuf (Vache) IgG.

Aperçu rapide pour IgG Kit ELISA (ABIN2043661)

Antigène: IgG

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Détail du produit IgG Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Sensibilité: < 0.29 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: High Sensitivity Immunoglobulin G (IgG) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G (IgG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G (IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G (IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Immunoglobulin G (IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IgG

Autre désignation: Immunoglobulin G (IgG)

Classe de substances: Antibody