HRH4 Kit ELISA

N° du produit ABIN2043695

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat HRH4.

Aperçu rapide pour HRH4 Kit ELISA (ABIN2043695)

Antigène: HRH4 (Histamine Receptor H4 (HRH4))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit HRH4 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.055 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Histamine Receptor H4 (HRH4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H4 (HRH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H4 (HRH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H4 (HRH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Histamine Receptor H4 (HRH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HRH4 (Histamine Receptor H4 (HRH4))