IRF4 Kit ELISA
Aperçu rapide pour IRF4 Kit ELISA (ABIN2043783)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.31 ng/mL
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Sensibilité
- < 0.133 ng/mL
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Plaque
- Pre-coated
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Protocole
- Interferon Regulatory Factor 4 (IRF4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 4 (IRF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 4 (IRF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 4 (IRF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interferon Regulatory Factor 4 (IRF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- IRF4 (Interferon Regulatory Factor 4 (IRF4))
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Pathways
- Chromatin Binding
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