IL-10RA Kit ELISA
Aperçu rapide pour IL-10RA Kit ELISA (ABIN2043797)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 78.12 pg/mL
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Sensibilité
- < 29 pg/mL
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Plaque
- Pre-coated
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Protocole
- Interleukin 10 Receptor Alpha (IL10Ra) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 Receptor Alpha (IL10Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 Receptor Alpha (IL10Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 10 Receptor Alpha (IL10Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
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Autre désignation
- Interleukin 10 Receptor alpha (IL10Ra)
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Pathways
- Growth Factor Binding
Antigène Voir toutes IL-10RA (IL10RA) Kits ELISA
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