IARS Kit ELISA

N° du produit ABIN2043823

Détail du produit IARS Kit ELISA

Antigène: IARS (Isoleucyl-tRNA Synthetase (IARS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Seuil minimal de détection: 0.31 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.108 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Isoleucyl tRNA Synthetase (IARS) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isoleucyl tRNA Synthetase (IARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isoleucyl tRNA Synthetase (IARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isoleucyl tRNA Synthetase (IARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Isoleucyl tRNA Synthetase (IARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IARS (Isoleucyl-tRNA Synthetase (IARS))

Autre désignation: Isoleucyl tRNA Synthetase (IARS) (IARS Produits)

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