Cytokeratin 19 Kit ELISA

N° du produit ABIN2043853

Kit ELISA Souris Cytokeratin 19, test Colorimetric pour la quantification de Souris Cytokeratin 19.

Aperçu rapide pour Cytokeratin 19 Kit ELISA (ABIN2043853)

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Application: ELISA

Détail du produit Cytokeratin 19 Kit ELISA

Seuil minimal de détection: 31.25 pg/mL

Sensibilité: < 13.6 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Keratin 19 (KRT19) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 19 (KRT19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 19 (KRT19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 19 (KRT19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 19 (KRT19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))