Keratin 33A Kit ELISA

N° du produit ABIN2043855

Le kit ELISA Keratin 33A (ABIN2043855) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Keratin 33A.

Aperçu rapide pour Keratin 33A Kit ELISA (ABIN2043855)

Antigène: Keratin 33A (KRT33A)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12-5000 pg/mL

Application: ELISA

Détail du produit Keratin 33A Kit ELISA

Seuil minimal de détection: 78.12 pg/mL

Sensibilité: < 29 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Keratin 33A (KRT33A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 33A (KRT33A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 33A (KRT33A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 33A (KRT33A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 33A (KRT33A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Keratin 33A (KRT33A)